山羊y+L碱性氨基酸转运系统转录水平表达的研究

doi:10.11843/j.issn.0366-6964.2015.08.005

Abstract

Abstract:

The objective of this study was to research the mRNA expression of goat 4F2hc，y⁺LAT1 and y⁺LAT2 (y⁺L cationic amino acid transporter system) in various tissues and their developmental expression in small intestine of goats at different ages.A total of 15 Shanbei White Cashmere goats at the ages of day 1，month 6，8，10 and 12 (3 goats at each age) were slaughtered to collect heart，liver，kidney，brain，muscle，skin，rumen，duodenum，jejunum，ileum and colon tissues.Then 4F2hc，y⁺LAT1 and y⁺LAT2 mRNA were quantified by Real-time quantitative PCR in all collected tissues of 1-day-old goats and in duodenum，jejunum，ileum of all goats.The results indicated that，(1) 4F2hc，y⁺LAT1 and y⁺LAT2 mRNA were widely expressed in all collected tissues of 1-day-old goats and the expression level varied from tissues.Specifically，4F2hc mRNA in skin，y⁺LAT1 mRNA in ileum and y⁺LAT2 mRNA in brain and ileum had the highest abundance.(2) 4F2hc mRNA of duodenum，jejunum and ileum on 1-day-old goats had higher abundance than that in goats on the other month-old，and there was no significant difference in the same segment of small intestine in goats on the other month-old (P>0.05)；y⁺LAT1 mRNA abundance in small intestine of goats decreased with age；y⁺LAT2 mRNA abundance in small intestine peaked in 10-month-old goats and then dropped with age，while it in ileum of 1-day-old goats had lower abundance than that in goats on the other month-old，and there was no significant difference in ileum between goats on the other month-old (P>0.05).In conclusion，y⁺L cationic amino acid transporter system played a main role in transferring amino acid in small intestine of goats and the heterodimer consisting of y⁺LAT1 and 4F2hc might be the main transporter protein in y⁺L system.4F2hc and y⁺LAT2 might perform important physiological functions in skin and brain of goats，respectively. 4F2hc，y⁺LAT1 and y⁺LAT2 were differentially regulated and distributed with the differences of developmental stages and segments of small intestine in goat.

CLC Number:

S827.2

ZHOU Ying-hao，GAO Ye，LIU Lin-li，ZENG Jie，YANG Yu-xin，ZHANG En-ping，CHEN Yu-lin. Study on Transcriptional Level of y⁺L Cationic Amino Acid Transporter System in Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1308-1316.

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Study on Transcriptional Level of y⁺L Cationic Amino Acid Transporter System in Goat

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Study on Transcriptional Level of y+L Cationic Amino Acid Transporter System in Goat

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Study on Transcriptional Level of y⁺L Cationic Amino Acid Transporter System in Goat